Rolling circle amplification and its application in microfluidic systems for Escherichia coli O157:H7 detections

• DOI: 10.1111/jfs.12671
• Publication Date: 2019-06-25T16:31:42Z
• Authors: Yuqian Jiang; Shuying Li; Zhenyu Qiu; Tao Le; Shan Zou; Xudong Cao
• Keywords: Rolling circle replication; SIGNAL (programming language)

Abstract Rolling circle amplification (RCA) has been widely used to enhance detection signals as its long single‐stranded RCA products can provide multiple binding sites for signal probes sensitive detections. In the current study, we employ atomic force microscopy (AFM) monitor during course of process over time. Subsequently, results obtained from AFM study are combined with those conventional electrophoresis method optimize reactions rapid and detection. We show that there appears be an inhomogeneous initiation phase in early mid‐stage reaction where some chains grow faster while others slower or remain dormant, observation not reported literature. Furthermore, demonstrate significantly by up 100‐fold. also Escherichia coli O157:H7 carried out different food matrices excellent sensitivities specificities. conclusion, these suggest a microfluidic device combination enhancement is simple robust approach whole‐cell samples. Practical applications The research exciting potentials detections samples safety inspections.