Innovations and stepwise evolution of CBFs/DREB1s and their regulatory networks in angiosperms

• DOI: 10.1111/jipb.13357
• Publication Date: 2022-09-07T17:23:23Z
• Authors: Yuqi Nie; Liangyu Guo; Fuqiang Cui; Yirong Shen; Xiaoxue Ye; Deyin Deng; Shuo Wang; Jianhua Zhu; Wenwu Wu
• Keywords: Neofunctionalization; Convergent evolution; Eudicots; Segmental duplication

The C-repeat binding factors/dehydration-responsive element protein 1s (CBFs/DREB1s) have been identified as major regulators of cold acclimation in many angiosperm plants. However, their origin and evolutionary process associated to responsiveness are still lacking. By integrating multi-omics data genomes, transcriptomes, CBFs/DREB1s genome-wide profiles, we unveil the evolution regulatory network. Gene collinearity phylogeny analyses show that CBF/DREB1 is an innovation evolved from tandem duplication-derived DREB III gene. A subsequent event ε-whole genome duplication led two archetypes (Clades I II) ancient angiosperms. In contrast cold-insensitivity Clade parent genes, II a further cold-sensitive response was stepwise expanded eudicots monocots by independent duplications. geological time, events were mainly enriched around Cretaceous-Paleogene (K-Pg) boundary and/or Late Cenozoic Ice Age, when global average temperature significantly decreased. Consequently, duplicated genes contributed rewiring CBFs/DREB1s-regulatory network for tolerance. Altogether, our results highlight convergent probably angiosperms adaptation cooling.