Three‐dimensional reconstruction of Haversian systems in human cortical bone using synchrotron radiation‐based micro‐CT: morphology and quantification of branching and transverse connections across age

• DOI: 10.1111/joa.12430
• Publication Date: 2016-01-10T09:10:57Z
• Authors: Isabel S. Maggiano; Corey M. Maggiano; John G. Clement; C. David L. Thomas; Yasmin Carter; David M. L. Cooper
• Keywords: Branching (polymer chemistry); Morphology; X-ray microtomography

This study uses synchrotron radiation-based micro-computed tomography (CT) scans to reconstruct three-dimensional networks of Haversian systems in human cortical bone order observe and analyse interconnectivity the development total across different ages. A better knowledge how interact with each other is essential improve understanding remodeling mechanisms maintenance; however, previous methodological approaches (e.g. serial sections) did not reveal enough detail follow specific morphology branching, for example. Accordingly, aim present was identify morphological diversity branching patterns transverse connections, understand they change age. Two types morphologies were identified: lateral resulting small osteon branches bifurcating off larger canals; dichotomous formation two new osteonal from one. The reconstructions this also suggest that frequently target previously existing as a path their course, cross-sectional referred 'type II osteons'. Transverse connections diverse course linear oblique curvy. Quantitative assessment age-related trends indicates while younger individuals most common, older more evidence growing inside found. Despite these changes characteristics, relatively constant degree overall maintained throughout life. Altogether, reveals important details about relation can be used towards well accurate interpretation variants osteons microscopy. Permitting visibility reversal lines, micro-CT valuable tool reconstruction systems, future analyses have potential further various aspects growth, maintenance health.