Botulinum toxin type A attenuates hypertrophic scar formation via the inhibition of TGF‐β1/Smad and ERK pathways

• DOI: 10.1111/jocd.13842
• Publication Date: 2020-11-13T15:50:05Z
• Authors: Yue‐Hua Li; Jiaomei Yang; Zhao Zheng; Da‐Hai Hu; Zhi‐Dong Wang
• Keywords: Hypertrophic scars; Type I collagen; Viability assay; Trypan blue

Abstract Background Hypertrophic scar is a common complication in would healing process, and how to effectively prevent treat it has been hot difficult research issue. Previous studies have showed that botulinum toxin type A (BTA) effects on the prevention treatment of hypertrophic scar, but little known about specific mechanisms. Objective This study aimed explore potential mechanisms BTA inhibition formation. Methods scar‐derived human fibroblasts were cultured then treated with transforming growth factor‐β1 (TGF‐β1) various concentrations BTA. Cell proliferation viability measured by CellTiter 96® AQueous One Solution Proliferation Assay trypan blue staining, respectively. The total amount collagen was examined using Sirius red staining. Collagen I III culture supernatant evaluated enzyme‐linked immunosorbent assay. Reverse transcription‐quantitative polymerase chain reaction Western blot analysis performed detect transcription translation levels. Results Our results revealed decreased fibroblasts. mRNA protein expression levels alpha‐smooth muscle actin, I, induced TGF‐β1 inhibited dose‐dependent manner. also phosphorylation Smad2/3 ERK. Conclusion prevented overdeposition ECM through TGF‐β1/Smad ERK pathways. findings this provide new scientific reference for scar.