LncRNA LINC00152 promotes oral squamous cell carcinoma growth via enhancing Upstream Transcription Factor 1 mediated Mitochondrial Ribosomal Protein L52 transcription

• DOI: 10.1111/jop.13253
• Publication Date: 2021-10-20T20:36:25Z
• Authors: Xiuhe Zou; Xiaokun Hu; Fenghui He; Ming Zhang; Xiangyu Kong; Shu Rui; Yang Liu; Liying Wang; Xun Zheng; Jiaye Liu; Zhihui Li; Han Luo
• Keywords: Mammals; Ribosomal Proteins; Transcription, Genetic; Squamous Cell Carcinoma of Head and Neck; 3. Good health; Gene Expression Regulation, Neoplastic; MicroRNAs; 03 medical and health sciences; 0302 clinical medicine; Head and Neck Neoplasms; Cell Line, Tumor; Carcinoma, Squamous Cell; Animals; Humans; Mouth Neoplasms; RNA, Long Noncoding; Hepatocyte Nuclear Factor 1-alpha; Cell Proliferation; Transcription Factors

LINC00152 (long intergenic non-protein coding RNA 152) was identified as an oncogenic lncRNA in multiple cancers. In the current study, we aimed to explore transcriptional profile of oral squamous cell carcinoma (OSCC) and its regulations at level.Bioinformatic analysis performed by extracting OSCC subset from The Cancer Genome Atlas (TCGA)-Head Neck Squamous Cell Carcinoma (HNSC). subcellular localization interacting factors (TFs) were explored. Dual-luciferase assay ChIP-qPCR applied study regulation. vitro vivo tumor growth models used for functional assays.NR_024206.2 dominant isoform that accounts 80% all transcripts LINC00152. upregulation associated with unfavorable survival patients OSCC. knockdown significantly impaired vivo. FISH confirmed nuclear cytoplasmic distribution It physically interacted Upstream Transcription Factor 1 (USF1), a common transcription factor mammalian cells. USF1 could bind promoter region MRPL52 (Mitochondrial Ribosomal Protein L52) activate transcription. enhance binding, thereby indirectly elevating expression. or slowed proliferation cells partly canceled LINC00152-mediated growth-promoting effects.This revealed novel LINC00152-USF1/MRPL52 axis promoting growth.