First report of Colletotrichum black leaf spot on strawberry caused by Colletotrichum siamense in China

• DOI: 10.1111/jph.13080
• Publication Date: 2022-03-03T13:52:40Z
• Authors: Cuicui Wang; Yuhai Tang; Ning Qiao; Dezhen Zhang; Wenjuan Chi; Jie Liu; Haoqin Pan; Jintang Li
• Keywords: Black spot; Spots; Colletotrichum; Fungal pathogen

Abstract In August 2020, a new fungal disease was observed on strawberry in Shandong Province, which showed that small spots of grayish‐black, near round without lesions and haloes the adaxial leaves. The morphological features colonies conida PDA medium were consistent with Colletotrichum gloeosporioides species complex. rDNA internal transcribed spacer, calmodulin , chitin synthase actin from isolates amplified sequenced. BLAST analysis these four genes 99.24‐100.00% identity corresponding sequences C. siamense GenBank. result phylogenetic also indicated pathogen identified as . Similar symptoms back leaf after spraying conidial suspensions for 3 days, reisolated confirm Koch's rule. This is first report black spot caused by