Analysis of differential metabolites of sunflower induced by virulent Verticillium dahlia V33 and hypovirulent Gibellulopsis nigrescens Vn‐1

• DOI: 10.1111/jph.13084
• Publication Date: 2022-02-28T14:18:15Z
• Authors: Jiao Peng; Bao‐Zhu Dong; Dong Wang; Ying‐Jie Zhao; Huan‐Wen Meng; Hong‐You Zhou
• Keywords: Jasmonic acid; Verticillium wilt; Phenylpropanoid

Abstract Verticillium wilt is a widespread soil‐borne vascular disease of crops. Hypovirulent strains pathogens show potential in providing cross‐protection against wilt. To identify metabolites associated with the induction resistance, gas chromatography–mass spectrometry‐based metabolomic technology was used to screen for differential induced by virulent ( dahlia V33) and hypovirulent Gibellulopsis nigrescens Vn‐1) pathogen sunflower Helianthus annuus ) roots. Forty‐eight were detected comparison between Vn‐1 V33 inoculation treatments. Among these metabolites, accumulation 17 secondary significantly higher response compared that after inoculation. We selected nine further analyses. Pyruvate, phenethylamine 2‐phenylbutyric acid inhibited infection Nicotiana benthamiana leaves Botrytis cinerea Phythophthora infestans . H 2 O burst at 24 hours post‐spray (hps) treatment N. organic acids. Quantitative RT‐PCR analysis revealed pyruvate, enhanced expression pathogenesis‐related genes hps, including PR1 , NPR1 PDF1.2 In addition, activities peroxidase, catalase, polyphenol oxidase superoxide dismutase increased. These results indicated many differentially involved resistance plant infection.