Transcriptome Analysis of Porphyromonas gingivalis Lipopolysaccharide‐Induced Early Gene Expression in Human Gingival Keratinocytes

• DOI: 10.1111/jre.13353
• Publication Date: 2024-10-16T13:29:56Z
• Authors: Mahyar Ostadkaram...; Edward E. Putnins
• Keywords: KEGG

ABSTRACT Aim Porphyromonas gingivalis lipopolysaccharide ( Pg LPS) is a significant virulence factor and driver of early innate immune responses in epithelial cells. The presence LPS immediate proximity to gingival epithelium induces inflammatory responses. In primary human keratinocytes (HGK), we utilized transcriptome analysis elucidate the change gene expression induced by LPS. Methods HGK cell cultures were treated with (4 h), RNA was extracted prepared for sequence (RNAseq) analysis. Differentially expressed genes (DEGs) identified, potential interactions between these subsequently examined using ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) analytic approaches identify significantly enriched pathways. Expression associated relevant pathways evaluated real‐time quantitative reverse‐transcription polymerase chain reaction (RT‐qPCR). Results RNAseq identified 25 DEGs, GO KEGG showed related two general First, broadly urokinase coagulation included PLAU , PLAUR SerpinB2 . RT‐qPCR analysis, over time (4–24 data consistent induction migration. Second, interleukin‐1 (IL‐1) receptor binding cytokine‐activity also enriched. IL36G IL1B IL1RN CXCL14 confirmed IL‐1family. When relation their respective antagonists, only increased. reduced time, this Conclusions are aspects periodontal disease (PDD) pathogenesis. changes an increase migration that found. both induced, but its selective antagonist IL36RN ) These support upregulation may serve as alarmin can drive HGK. Further vivo testing findings ongoing.