Virulence profile ofEscherichia coliO157 strains isolated from surface water in cattle breeding areas

• DOI: 10.1111/lam.12873
• Publication Date: 2018-03-03T03:04:37Z
• Authors: J.D. Tanaro; L.A. Pianciola; B.A. D'Astek; M.C. Piaggio; M.L. Mazzeo; G. Zolezzi; M. Rivas
• Keywords: Persistence (discontinuity)

Shiga toxin‐producing Escherichia coli (STEC) O157:H7 is a worldwide concern. Cattle are their main reservoir and may contaminate watercourses through manure. We characterized collection of 38 STEC strains isolated from surface water in feedlots areas (puddles inside pens formed after the rainfall or by spill around drinking troughs, small courses lagoons, runoff). Nineteen (50·0%) harboured stx2a/stx2c genes, 18 (47·4%) stx2c one stx1a/stx2c. All eae, ehxA, rfbO157 fliCH7 putative virulence determinants ECSP_0242, ECSP_2687 ECSP_3620. isolates tested as Lineage I/II lineage‐specific polymorphism assay‐6. (50%) belonged to high virulent clade 8. The q21 allele was found all q933/q21 alleles 17 (44·7%). By XbaI‐pulsed‐field gel electrophoresis, 29 were grouped into seven clusters. Four clusters distant places separated 150–250 km. This be related vectors, like birds, involved spread. Otherwise, three contained recovered at same with intervals 1–9 months. could explained environmental persistence O157:H7. These showed similar genotypes those bovine human diseases, linked incidence haemolytic uremic syndrome Argentina. challenge for growing global demand food find sustained production strategies without collateral effects. Intensive livestock operations generate large volumes manure that can finite resource, water. study shows how contaminated confined feeding transport dangerous pathogens warns pay more attention control sanitation systems prevent this type pollution.