Structural basis for arabinoxylo‐oligosaccharide capture by the probiotic Bifidobacterium animalis subsp. lactis Bl‐04

• DOI: 10.1111/mmi.12419
• Publication Date: 2013-10-31T10:30:11Z
• Authors: Morten Ejby; Folmer Fredslund; Andreja Vujicic‐Z...; Birte Svensson; Dirk Jan Slotboom; Maher Abou Hachem
• Keywords: Bifidobacterium animalis; Oligosaccharide

Summary Glycan utilization plays a key role in modulating the composition of gut microbiota, but molecular insight into oligosaccharide uptake by this microbial community is lacking. Arabinoxylo‐oligosaccharides ( AXOS ) are abundant diet, and selectively fermented probiotic bifidobacteria colon. Here we show how selectivity for established strain B ifidobacterium animalis subsp. lactis Bl ‐04. The binding protein AXBP , which associated with an ATP ‐binding cassette ABC transporter that mediates displays exceptionally broad specificity arabinosyl‐decorated undecorated xylo‐oligosaccharides, preference tri‐ tetra‐saccharides. Crystal structures complex four different ligands revealed basis versatility. Uniquely, was able to recognize oligosaccharides two opposite orientations, facilitates optimization interactions various ligands. Broad substrate further enhanced spacious pocket accommodating decorations at mainchain positions conformational flexibility lid‐like loop. Phylogenetic genetic analyses highly conserved within lacking other microbiota members. These data indicate niche adaptation highlight metabolic syntrophy (cross‐feeding) among microbiota.