Nuclear localization of a putative Phytophthora sojae bZIP1 transcription factor is mediated by multiple targeting motifs

• DOI: 10.1111/mmi.13652
• Publication Date: 2017-02-18T08:26:49Z
• Authors: Yufeng Fang; Brett M. Tyler
• Keywords: NLS; Phytophthora sojae; Oomycete; Transcription

Oomycetes are fungal-like eukaryotic microbes in the kingdom Stramenopila. We recently found that oomycete plant pathogen Phytophthora sojae uses nuclear localization signals (NLSs) for translocation of proteins into nucleus differ from conventional well-characterized NLSs mammals and yeast. Here, we have characterized depth a P. basic leucine zipper transcription factor, PsbZIP1. Nuclear PsbZIP1 was determined by central conserved region overlapping DNA binding domain. Mutational analysis this identified four distinct elements contributed multiplicatively to localization, but residues were not required. Three showed autonomous NLS activity fourth served as enhancer. Sequences within two defined new form bipartite consisting triplet followed tail scattered amino acids.