FtsW activity and lipid II synthesis are required for recruitment of MurJ to midcell during cell division in Escherichia coli

• DOI: 10.1111/mmi.14104
• Publication Date: 2018-08-16T03:38:42Z
• Authors: Xiaolong Liu; Nils Y. Meiresonne; Ahmed Bouhss; Tanneke den Blaauwen
• Keywords: Lipid II; Cell envelope; Cell membrane

Summary Peptidoglycan (PG) is the unique cell shape‐determining component of bacterial envelope, and a key target for antibiotics. PG synthesis requires transmembrane movement precursor lipid II, MurJ has been shown to provide this activity in Escherichia coli . However, how functions vivo not reported. Here we show that localizes both lateral membrane at midcell, recruited midcell simultaneously with late‐localizing divisome proteins MraY MurG. septal localization dependent on presence complete active divisome, II PBP3/FtsW activities. Inactivation MurJ, either directly by mutation or through binding MTSES, did affect MurJ. Our study visualizes reveals possible mechanism recruitment during division.