Pseudouridines on Trypanosoma brucei mRNAs are developmentally regulated: Implications to mRNA stability and protein binding

• DOI: 10.1111/mmi.14774
• Publication Date: 2021-06-24T13:42:36Z
• Authors: K. Shanmugha Rajan; Katerina Adler; Hava Madmoni; Dana Peleg‐Chen; Smadar Cohen‐Chal...; Tirza Doniger; Beathrice Galili; Doron Gerber; Ron Unger; Christian Tschudi; Shulamit Michaeli
• Keywords: 0301 basic medicine; RNA Stability; Trypanosoma brucei brucei; RNA-Binding Proteins; High-Throughput Screening Assays; 03 medical and health sciences; Gene Expression Regulation; Animals; RNA, Messenger; 3' Untranslated Regions; Intramolecular Transferases; Pseudouridine; Protein Binding

AbstractThe parasite Trypanosoma brucei cycles between an insect and a mammalian host and is the causative agent of sleeping sickness. Here, we performed high‐throughput mapping of pseudouridines (Ψs) on mRNA from two life stages of the parasite. The analysis revealed ~273 Ψs, including developmentally regulated Ψs that are guided by homologs of pseudouridine synthases (PUS1, 3, 5, and 7). Mutating the U that undergoes pseudouridylation in the 3′ UTR of valyl‐tRNA synthetase destabilized the mRNA level. To investigate the mechanism by which Ψ affects the stability of this mRNA, proteins that bind to the 3′ UTR were identified, including the RNA binding protein RBSR1. The binding of RBSR1 protein to the 3′ UTR was stronger when lacking Ψ compared to transcripts carrying the modification, suggesting that Ψ can inhibit the binding of proteins to their target and thus affect the stability of mRNAs. Consequently, Ψ modification on mRNA adds an additional level of regulation to the dominant post‐transcriptional control in these parasites.