A CysB regulator positively regulates cysteine synthesis, expression of type III secretion system genes, and pathogenicity in Ralstonia solanacearum

• DOI: 10.1111/mpp.13189
• Publication Date: 2022-02-05T04:43:33Z
• Authors: Min Chen; Weiqi Zhang; Liangliang Han; Xuejuan Ru; Yuzhu Cao; Yasufumi Hikichi; Kouhei Ohnishi; Guanghui Pan; Yong Zhang
• Keywords: Ralstonia solanacearum; Regulon; Bacterial wilt

A syringe-like type III secretion system (T3SS) plays essential roles in the pathogenicity of Ralstonia solanacearum, which is a causal agent bacterial wilt disease on many plant species worldwide. Here, we characterized functional CysB regulator (RSc2427) R. solanacearum OE1-1 that was demonstrated to be responsible for cysteine synthesis, expression T3SS genes, and solanacearum. The cysB mutants were auxotrophs failed grow minimal medium but grew slightly host plants. Supplementary substantially restored impaired growth both inside Genes cysU cysI regulons have been annotated function synthesis; positively regulated these genes. Moreover, genes vitro planta through PrhG HrpB pathway, whilst independent deficiency under nutrient-limited conditions. also required exopolysaccharide production swimming motility, contribute jointly colonization infection process Thus, identified here as novel These results provide insights into understanding various biological functions regulators complex regulatory networks