The novel avirulence effector AlAvr1 from Ascochyta lentis mediates host cultivar specificity of ascochyta blight in lentil

• DOI: 10.1111/mpp.13203
• Publication Date: 2022-03-05T05:30:25Z
• Authors: Bernadette M. Hen...; Johannes W. Debler; Lina M. Farfan‐Ca...; Christina R. Grime; Robert A. Syme; Sara N. Blake; Herdina; Jennifer A. Davidson; Richard P. Oliver; Karam B. Singh; Lars G. Kamphuis; Robert C. Lee
• Keywords: Ascochyta; Pathosystem

Abstract Ascochyta lentis is a fungal pathogen that causes ascochyta blight in the important grain legume species lentil, but little known about molecular mechanism of disease or host specificity. We employed map‐based cloning approach using biparental A . population to clone gene AlAvr1‐1 encodes avirulence towards lentil cultivar PBA Hurricane XT. The mapping was produced by mating isolate P94‐24, which pathogenic on Nipper and avirulent Hurricane, Al Kewell, not Nipper. Using agroinfiltration, we found from P94‐24 necrosis homologous corresponding AlAvr1‐2 , protein with amino acid variation at 23 sites four these have been positively selected branch phylogeny. Loss knockout experiment mutant strain virulent Hurricane. Deletion Kewell led reduced pathogenicity suggesting may contribute did affect virulence for therefore an conclude hemibiotrophic has effector, AlAvr1‐1, triggers hypersensitive resistance response This first be characterized Pleosporales help progress research other damaging pathogens.