EndogenousCRFin rat large intestine mediates motor and secretory responses to stress

• DOI: 10.1111/nmo.12725
• Publication Date: 2015-11-27T01:49:15Z
• Authors: S. Liu; J. Chang; N. Long; K. Beckwith; G. Talhouarne; J. J. Brooks; M.‐H. Qu; W. Ren; J. D. Wood; S. Cooper; A. Bhargava
• Keywords: Male; Restraint, Physical; Colon; Corticotropin-Releasing Hormone; Clinical Sciences; Medical Physiology; Clinical sciences; Enzyme-Linked Immunosorbent Assay; Restraint; Stress; Polymerase Chain Reaction; Rats, Sprague-Dawley; stress; 03 medical and health sciences; Medical physiology; Genetics; Physical; Animals; 0303 health sciences; Biomedical and Clinical Sciences; colon; Gastroenterology & Hepatology; Animal; Prevention; Neurosciences; Pharmacology and Pharmaceutical Sciences; CRF; Immunohistochemistry; Rats; Disease Models, Animal; motility; Disease Models; ion secretion; Psychological; Sprague-Dawley; Digestive Diseases; Gastrointestinal Motility; Stress, Psychological

Abstract Background Corticotropin‐releasing factor ( CRF ) mediates our body's overall responses to stress. The role of central in stress‐stimulated colonic motility is well characterized. We hypothesized that transient perturbation expression enteric sufficient change stress‐induced motor and secretory responses. Methods Sprague–Dawley rats (adult, male) were subjected 1‐h partial restraint stress PRS euthanized at 0, 4, 8, 24 h. mRNA peptide levels the colon quantified by real‐time RT ‐ PCR , enzyme immuno‐assay immunohistochemistry. Double‐stranded RNA (ds designed target was injected into wall attain interference‐mediated inhibition expression. Ds for β ‐globin used as a control (dsControl). Four days after ds injection, . Fecal output measured. Ussing chamber techniques assess mucosal ion secretion transepithelial tissue conductance. Key Results Exposure elevated increased release rat colon. Injection inhibited basal prevented ‐induced increase expression, whereas dsControl‐injected colons remained high In treated with dsControl, caused significant fecal pellet output, baseline secretion, Inhibition Conclusions & Inferences These results provide direct evidence peripherally expressed prevents