A putative anti‐inflammatory role for TRPM8 in irritable bowel syndrome—An exploratory study

• DOI: 10.1111/nmo.14170
• Publication Date: 2021-06-19T11:50:33Z
• Authors: Madusha Peiris; Zsa Zsa R. M. Weerts; Rubina Aktar; Ad A. M. Masclee; Ashley Blackshaw; Daniel Keszthelyi
• Keywords: TRPM8

Abstract Background Chronic and recurring pain is a characteristic symptom in irritable bowel syndrome (IBS). Altered signaling between immune cells sensory neurons within the gut may promote generation of symptoms. As transient receptor potential melastatin 8 (TRPM8) agonists, such as L‐menthol peppermint oil, have shown to attenuate IBS symptoms, we began investigating molecular mechanisms. Methods Colonic biopsy tissues were collected from patients with controls, two separate cohorts. Immunohistochemistry was performed identify TRPM8 localization. Quantitative PCR measure mucosal mRNA levels TRPM8. In addition, functional experiments agonist icilin ex vivo examine cytokine release biopsies. Daily diaries ascertain Results tissue patients, showed that immunoreactivity colocalized predominantly dendritic cell lineage, close approximation nerve endings, protein expression increased compared controls ( p < 0.001). significant positive association abdominal scores = 0.015). Treatment patient biopsies reduced inflammatory cytokines IL‐1β, IL‐6, TNF‐α 0.05). Conclusions inferences These data indicate important anti‐inflammatory properties by this virtue can impact neuro‐immune disease mechanisms IBS.