Dissecting the components controlling root‐to‐shoot arsenic translocation in Arabidopsis thaliana

• DOI: 10.1111/nph.14761
• Publication Date: 2017-08-31T12:05:43Z
• Authors: Chengcheng Wang; GunNam Na; Eduardo Sanchez B...; Yi Chen; Jo Ann Banks; David E. Salt; Fang‐Jie Zhao
• Keywords: 0106 biological sciences; tolerance; Arabidopsis thaliana; Arsenate Reductases; Arabidopsis Proteins; arsenic accumulation; arsenite efflux; arsenic; Arabidopsis; arsenate reductase; Biological Transport; Pteris; Pteris vittata; Aminoacyltransferases; Plant Roots; 01 natural sciences; Arsenic; Gene Knockout Techniques; Mutation; Plant Shoots; Plant Proteins

Summary Arsenic (As) is an important environmental and food‐chain toxin. We investigated the key components controlling As accumulation tolerance in Arabidopsis thaliana . tested effects of different combinations gene knockout, including arsenate reductase ( HAC 1 ), γ‐glutamyl‐cysteine synthetase (γ ‐ ECS phytochelatin synthase PCS ) phosphate effluxer PHO heterologous expression As‐hyperaccumulator Pteris vittata arsenite efflux Pv ACR 3 on tolerance, accumulation, translocation speciation A. Heterologous markedly increased root‐to‐shoot , with being localized to plasma membrane. Combining mutation led hyperaccumulation shoots, whereas combining decreased accumulation. Mutants γ‐ were hypersensitive had higher translocation. or did not alter beyond levels observed single mutants. have large can be engineered by knocking out expressing also affect but extent 1.