Cyclophilin effector Al106 of mirid bug Apolygus lucorum inhibits plant immunity and promotes insect feeding by targeting PUB33

• DOI: 10.1111/nph.18675
• Publication Date: 2022-12-15T07:50:41Z
• Authors: Yumei Dong; Jiangxuan Zhou; Yuxia Yang; Wangshan Lu; Yan Jin; Xingge Huang; Wendan Zhang; Jifen Li; Gan Ai; Zhiyuan Yin; Danyu Shen; Maofeng Jing; Daolong Dou; Ai Xia
• Keywords: Plant Immunity; Plant protein

Summary Apolygus lucorum (Meyer‐Dur; Heteroptera: Miridae) is a major agricultural pest infesting crops, vegetables, and fruit trees. During feeding, A. secretes plethora of effectors into its hosts to promote infestation. However, the molecular mechanisms these manipulating plant immunity are largely unknown. Here, we investigated mechanism underlying effector Al106 manipulation plant–insect interaction by RNA interference, electrical penetration graph, insect pathogen bioassays, protein–protein studies, protein ubiquitination experiment. Expression in Nicotiana benthamiana inhibits pathogen‐associated pattern‐induced cell death reactive oxygen species burst, promotes feeding infection. In addition, peptidyl‐prolyl cis‐trans isomerase (PPIase) activity required for function inhibit PTI.Al106 interacts with U‐box (PUB) protein, PUB33, from N. Arabidopsis thaliana . We also demonstrated that PUB33 positive regulator immunity. Furthermore, an vivo assay revealed NbPUB33 depending on PPIase activity. Our findings novel cyclophilin may interact suppress facilitate activity‐dependent manner.