A land plant‐specific VPS13 mediates polarized vesicle trafficking in germinating pollen

• DOI: 10.1111/nph.20277
• Publication Date: 2024-12-02T03:00:11Z
• Authors: Surachat Tangpran...; Yuka Kimura; Motoko Igarashi; Fumiko Ishizuna; Yoshinobu Kato; Takamasa Suzuki; Takuya Nagae; Sota Fujii; Seiji Takayama
• Keywords: Sporopollenin; Double fertilization; Tip growth

Summary Pollen has an extraordinary ability to convert from a dry state extremely rapidly growing state. During pollination, pollen receives water and Ca 2+ the contacting pistil, which will be directional cue for tube germination. The subsequent rapid activation of vesicular transport must support growth, but molecular mechanism leading this process is largely unknown. We established luciferase‐based pollination assay screen genetic mutants defective in early stage after pollination. identified plant‐specific VPS13, Arabidopsis thaliana VPS13a as important germination, studied its function. AtVPS13a mutation severely affected germination lipid droplet discharge rough endoplasmic reticulum. Cellular accumulation patterns secretory vesicle marker were synchronized at polarized site, with slight delay local elevation. found brief spike initiation hydration, may related cues emergence. Although dynamics was unaffected by absence AtVPS13a, mutant suffered reduced cell wall deposition during mediates polarization, regulating proper following signaling outgrowth.