Integrated Analysis of Oral Microbiome and Metabolome in T2DM Patients With Varying Glycemic Status

• DOI: 10.1111/odi.15220
• Publication Date: 2024-12-17T10:54:52Z
• Authors: Jing Diao; Yanling Zhang; Xiaoli Zhang; Shuyuan Jia; Yajuan Lei; Bowen Ma; Xiaodong Li; Shuguo Zheng; Chao Yuan
• Keywords: Metabolome; Veillonella; Oral microbiology

ABSTRACT Objective This study aimed to analyze the subgingival and salivary microbiome metabolome in diabetic periodontitis patients with varying glycemic levels. Methods Forty‐two were sampled of saliva, gingival crevicular fluid (GCF), blood, categorized into three groups based on systemic status. The was assessed using full‐length 16S rRNA gene sequencing. Gas chromatography–mass spectrometry performed for analysis. Results similarity structure function flora saliva GCF evident under good blood glucose control. Conversely, inadequate control demonstrated a more distinct separation from flora. Both microorganisms exhibited greater periodontal pathogenicity, metabolites showing stronger associations inflammation when Hemoglobin A1c (HbA1c) > 6.5%. Some pathogens, such as Veillonella atypica , showed significantly positive correlations proinflammatory metabolites, including lactic acid putrescine, etc. Salivary microbes sensitive responses than changes levels among type 2 diabetes mellitus (T2DM) patients. Conclusion Under active control, it indicates lower pathogenicity inflammatory correlation oral microecology T2DM Saliva appears offer superior diagnostic monitoring value compared context disease surveillance.