An insertion of transposon in DcNAP inverted its function in the ethylene pathway to delay petal senescence in carnation (Dianthus caryophyllus L.)

• DOI: 10.1111/pbi.14132
• Publication Date: 2023-08-26T04:51:24Z
• Authors: Zheng Sun; Manman Wu; Siqi Wang; Shan Feng; Yan Wang; Teng Wang; Chunlin Zhu; Xinyu Jiang; Hongya Wang; Ruiming Wang; Xinyi Yuan; Menglu Wang; Linlin Zhong; Yunjiang Cheng; Manzhu Bao; Fan Zhang
• Keywords: Dianthus; Petal; Transcription

Petal senescence is the final stage of flower development. Transcriptional regulation plays key roles in this process. However, whether and how post-transcriptional involved still largely unknown. Here, we identified an ethylene-induced NAC family transcription factor DcNAP carnation (Dianthus caryophyllus L.). One allele, DcNAP-dTdic1, has insertion a dTdic1 transposon its second exon. The disrupts structure causes alternative splicing, which transcribes multiple domain-deleted variants (DcNAP2 others). Conversely, wild type allele DcNAP1 encoding intact domain. Silencing delays overexpressing accelerates petal carnation, while silencing DcNAP2 have opposite effects, respectively. Further, could interact with interfere binding activation activity to promoters downstream target ethylene biosynthesis genes DcACS1 DcACO1. Lastly, signalling core transcriptional DcEIL3-1 can activate expression same way by their promoters. In summary, discovered novel mechanism regulates at level. It may also provide useful strategy manipulate domains factors for crop genetic improvement.