Beyond species and spatial boundaries: Enabling long‐distance gene silencing in plants via guanidinium‐siRNA nanoparticles

• DOI: 10.1111/pbi.14575
• Publication Date: 2025-02-07T11:39:52Z
• Authors: Shujin Lin; Qian Zhang; Shiyan Bai; Liwen Yang; Guannan Qin; Liyuan Wang; Wenbin Wang; Cui Cheng; Da Zhang; Chunhua Lu; Jifeng Yuan; Jingying Li; Huanghao Yang; Xiaofeng Gu; Xiao Han

Summary RNA interference (RNAi) has been widely used in agriculture. However, it is well accepted that common methods of plant RNAi are species‐dependent and lack systematic efficiency. This study designed a thiolated siRNA nanoparticle, guanidinium (Gu + )‐containing disulfide assembled ‐siRNA), demonstrating remarkable species independence efficient systemic gene silencing across different species. Our results indicate this approach effectively utilizes the vascular system to deliver siRNA, enabling long‐distance both monocot dicot plants, such as rice Arabidopsis. By applying method, we successfully targeted silenced key genes like STM , WER MYB23 GD1 EIL1 EIL2 which regulate development enhance salt tolerance. delivery significantly expands application technology serving valuable tool for advancing agricultural biotechnology, enhancing crop resistance, improving productivity, while aligning with global goals sustainable food production improvement.