Calcium‐dependent protein kinase PpCDPK29‐mediated Ca2+‐ROS signal and PpHSFA2a phosphorylation regulate postharvest chilling tolerance of peach fruit

• DOI: 10.1111/pbi.70024
• Publication Date: 2025-03-10T07:22:00Z
• Authors: Liangyi Zhao; Hua Cassan‐Wang; Yaqin Zhao; Yinqiu Bao; Yuanyuan Hou; Yu Liu; Zhengguo Wu; Mondher Bouzayen; Yonghua Zheng; Peng Jin

Summary Green and chemical‐free hot water (HW) treatment can effectively reduce the chilling injury of peach fruit; however, mechanism inducing resistance by heat is still unclear. This study found that HW could activate reactive oxygen species (ROS) signalling, forming ROS‐Ca 2+ signalling. Furthermore, we identified a Ca sensor, calcium‐dependent protein kinase 29 (PpCDPK29), as positive regulator postharvest resistance. PpCDPK29 interacted with ROS‐generating proteins (PpRBOHC/D) antioxidant enzymes (PpSOD PpCAT1) to jointly maintain ROS homeostasis. Meanwhile, PpHSFA2a was phosphorylated transferred nucleus, which enhanced binding ability target genes. Here, activated transcription genes PpHSP18.5 , PpHSP70 PpGSTU7 PpGSTU19 PpGolS1 PpBAM1 acted molecular chaperones, improved scavenging osmoregulation alleviate fruit. In summary, in fruit activating PpCDPK29‐mediated ‐ROS HSF‐HSP signalling pathways, providing novel network for quality control