Temporal kinetics of the transcriptional response to carbon depletion and sucrose readdition in Arabidopsis seedlings

• DOI: 10.1111/pce.12642
• Publication Date: 2015-09-19T15:04:59Z
• Authors: Sarah Jane Cookson; Umesh Prasad Yadav; Sebastian Klie; Rosa Morcuende; Björn Usadel; John Edward Lunn; Mark Stitt
• Keywords: Carbon fibers

Abstract To investigate whether the transcriptional response to carbon (C) depletion and sucrose resupply depends on duration severity of C depletion, Arabidopsis seedlings were grown in liquid culture harvested 3, 6, 12, 24, 48 72 h after removing from medium 30 min resupplying at each time. Expression profiling revealed early inhibition cell wall synthesis remodelling signalling, followed by induction recycling photosynthesis general growth. The temporal sequence differed published progressive exhaustion during a night extended vegetatively growing plants. readdition was conserved across C‐depletion time course. Intriguingly, vast majority rapidly responding transcripts decreased rather than increased. that respond many also decrease treating with inhibitor cordycepin A. Comparison responses overexpression otsA , AKIN10 bZIP11 genes especially resupply, one or more these C‐signalling components. Thus, multiple factors contribute responsiveness, including signalling components, regulation transcript turnover.