When rice gets the chills: comparative transcriptome profiling at germination shows WRKY transcription factor responses

• DOI: 10.1111/plb.13262
• Publication Date: 2021-03-27T01:59:40Z
• Authors: V. E. Viana; L. Carlos da Maia; C. Busanello; C. Pegoraro; A. Costa de Oliveira
• Keywords: WRKY protein domain; RNA-Seq; RNA polymerase II

Abstract Rice is vital for food security. Due to its tropical origin, rice suffers from cold temperatures that affect entire life cycle. Key genes have been identified involved in tolerance. WRKYs are generally downstream of the MAPK cascade and can act together with VQ proteins regulate stress‐responsive genes. Chilling treatment was applied at germination two genotypes (tolerant sensitive). Shoots S3 stage were collected RNA‐seq identify OsWRKY , OsMAPKs OsVQs expression. Relationships among MAPKs, VQs predicted through correlation analysis. transcriptional regulation by silico analysis cis ‐regulatory elements. A total 39 s differentially expressed. OsWRKY21 OsWRK24 OsWRKY69 potential positive regulators, while OsWRKY10 OsWRK47, OsWRKY62, OsWRKY72 OsWRKY77 negative chilling 12 downregulated negatively correlated upregulated OsWRKYs tolerant genotype. 19 expressed, three six positively sensitive genotypes, respectively. Seven expressed cold‐responsive elements their promoters five genotype contained W‐box motif. causes changes OsMAPK OsVQ gene expression germination. may not coordinate tolerance, but Candidate a promoter, suggesting an auto‐regulation mechanism.