The loss‐of‐function of AtNATA2 enhances AtADC2‐dependent putrescine biosynthesis and priming, improving growth and salinity tolerance in Arabidopsis

• DOI: 10.1111/ppl.14603
• Publication Date: 2024-11-04T02:40:26Z
• Authors: Francisco Ignacio...; Carlos Eduardo Au...; Sanja Ćavar Zeljk...; Iñigo Saiz‐Fernández; Pavel Klimeš; Nuria De Diego
• Keywords: Polyamine; Crosstalk

Abstract Putrescine (Put) is a promising small molecule‐based biostimulant to enhance plant growth and resilience, though its mode of action remains unclear. This study investigated the Put priming effect on Arabidopsis mutant lines ( Atadc1, Atadc2, Atnata1 , Atnata2 ) under control conditions salinity understand role in regulating growth. The Atadc2 mutant, characterized by reduced endogenous levels, showed insensitivity without enhancement, which was linked significant imbalances nitrogen metabolism, including high Gln/Glu ratio. Contrarily, exhibited improvement upregulated AtADC2 expression, particularly priming, highlighting these genes' involvement development. enhanced inducing accumulation specific polyamines (free, acetylated, conjugated, or bound form) improving light‐harvesting efficiency, line. Our findings suggest that At NATA2 may negatively regulate synthesis via ADC2 chloroplast, impacting light harvesting photosystem II (PSII). Furthermore, line AtADC1 but AcPut pointing cross‐regulation among genes. regulation ADC1 could be crucial for overall stress tolerance interacting with polyamine catabolism, shapes metabolic profile different conditions. Understanding regulatory mechanisms involving crosstalk between AtADC AtNATA genes metabolism connection certain SMBBs like can lead more effective agricultural practices, growth, uptake, resilience challenging