CK1 inhibitor affects in vitro maturation and developmental competence of bovine oocytes

• DOI: 10.1111/rda.13483
• Publication Date: 2019-06-03T02:33:15Z
• Authors: Pengfei Shi; Jie Xu; Xin Zhao; Penglei Shen; Dongmei Wen; Qing Yu; Yanfei Deng; Deshun Shi; Fenghua Lu
• Keywords: In vitro maturation; Germinal vesicle

The objectives of present study were to evaluate the effect casein kinase 1 (CK1) inhibition D4476 on in vitro maturation (IVM) and developmental competence bovine oocytes. cumulus oocyte complexes (COCs) cultured medium with (0, 2, 5, 10, 20 μM) for 24 hr. After IVM fertilization, through expansion average scores cells (CCs), efficiency, cleavage rate blastocyst zygote, we found 5 μM could increase development potential COCs treated D4476, results quantitative real-time PCR analysis, Lichen red staining PI showed that under without affecting germinal vesicle breakdown nuclear morphology, significantly decrease CK1 upregulate TCF-4 Furthermore, influencing level Bad CTSB, expression β-catenin, TCF-4, Cx43, MAPK, PTGS-2, PTX-3, TGS-6, Bax Bcl-2 CCs. Western blot analysis revealed addition during resulted a lower Cx43 protein at 12 hr higher compared group D4476. These indicate adding optimum (5 is beneficial maturity efficiency