The presence of duck Tembusu virus in Thailand since 2007: A retrospective study

• DOI: 10.1111/tbed.12859
• Publication Date: 2018-03-09T04:08:53Z
• Authors: P. Ninvilai; N. Nonthabenjawan; B. Limcharoen; W. Tunterak; K. Oraveerakul; W. Banlunara; A. Amonsin; A. Thontiravong
• Keywords: Flavivirus

Duck Tembusu virus (DTMUV), a newly emerging in ducks, was first reported China 2010. However, an unknown severe contagious disease associated with neurological signs and egg production losses resembling to DTMUV infection, observed Thailand since 2007. To determine the presence of 2007, clinical samples from affected ducks collected 2007 were tested for using pathological virological analyses. Gross histopathological lesions mostly restricted ovary, brain spinal cord, correlated flavivirus antigen cord samples. Subsequently, identified by RT-PCR nucleotide sequencing polyprotein gene. Phylogenetic analysis gene sequence revealed that Thai unique virus, belonged within cluster 1, but distinctively separated Malaysian DTMUV, which most closely related DTMUV. It is interesting note genetically different currently circulating Chinese DTMUVs, 2. Our findings indicated emerged earlier common ancestor recently DTMUVs; however, it distinctive any DTMUVs. In conclusion, our data demonstrated prior report This study indicates may have circulated region long before 2010 highlights high genetic diversity DTMUVs Asia.