A practical guide for strategic and efficient sampling in African swine fever‐affected pig farms

• DOI: 10.1111/tbed.14582
• Publication Date: 2022-05-03T09:59:33Z
• Authors: Kristīne Lamberga; Klaus Depner; Laura Zani; Edvīns Oļševskis; Mārtiņš Seržants; Santa Ansonska; Žanete Šteingolde; Aivars Bērziņš; Arvo Viltrop; Sandra Blome; Anja Globig
• Keywords: Culling; Biosecurity; African Swine Fever; Pig farming; Classical swine fever

In the case of African swine fever (ASF) outbreaks in pig farms, EU legislation requires a thorough epidemiological investigation to determine, among other tasks, extent infection affected farm. The main aim this study was implement reliable sampling strategy quickly obtain an overview ASF virus spread We developed and tested three-step approach: (i) identification sub-units within farm, (ii) categorization sub-units, (iii) targeted selection animals for testing. used commercially available lateral flow devices (LFDs) detect antigen antibodies under field conditions compared them with routinely performed laboratory tests (qPCR, ELISA, IPT). conducted three commercial farms Latvia that were by July 2020. One relatively small only 31 pigs, whereas two large 1800 9800 animals, respectively. approach proved be helpful practical efficient reliably assess situation on farm identify where infected are present which might (still) free infection. This important information helps better estimate high-risk period track potential outside It allows also prioritize culling and, if appropriate, pursue partial taking into account absence clinical signs, implemented biosecurity measures, quarantine negative test results, others. interest identified very early has not yet widely. Due its limited sensitivity, LFD is useful testing showing signs disease.