Preliminary survey of domestic animal visceral leishmaniasis and risk factors in north‐west Ethiopia

• DOI: 10.1111/tmi.12418
• Publication Date: 2014-10-20T03:14:58Z
• Authors: Ambaye Kenubih; Shimelis Dagnachew; Gizat Almaw; Tamerat Abebe; Yegnasew Takele; Asrat Hailu; Wessensegad Lemma
• Keywords: Direct agglutination test; Giemsa stain; Canine leishmaniasis

Abstract Objective After the epidemics of L. donovani complex in 2004/05 human patients, to investigate presence antibodies against domestic animals north‐west Ethiopia. Methods Two hundred and three were screened. Serum biopsy samples collected. A modified direct agglutination test ( DAT ) for canine reservoirs was used screen serum at ≥ 1:320 cut‐off titre. Giemsa stain culture on Novy macNeal Nicolae NNN media samples. Pre‐tested questionnaires elicit information potential risk factors. Results Antibody detected 30.5% animals. The highest seropositivity rates 41.9% cattle, 40% dogs, 33.3% donkeys, 10% goats 4.8% sheep. No Leishmania parasite isolated from spleen, liver, skin snip exudates, bone marrow or lymph node dogs. Dogs owned by households with history kala‐azar treatment humans sharing house cattle more affected visceral leishmaniasis P < 0.05). Conclusion This study showed a high serological prevalence Their role epidemiology remains unclear.