C2‐mediated decrease in DNA methylation, accumulation of siRNAs, and increase in expression for genes involved in defense pathways in plants infected with beet severe curly top virus

• DOI: 10.1111/tpj.12081
• Publication Date: 2012-11-23T17:55:11Z
• Authors: Li‐Ping Yang; Yuan‐Yuan Fang; Chun‐Peng An; Li Dong; Zhong‐Hui Zhang; Hao Chen; Qi Xie; Hui‐Shan Guo
• Keywords: RNA-Directed DNA Methylation

Cytosine methylation is one of epigenetic information marked on the DNA sequence. In plants, small interfering RNAs (siRNAs) target homologous genomic sequences for cytosine methylation. This process, known as RNA-directed (RdDM), plays an important role in transposon control, regulation gene expression and virus resistance. this paper, we demonstrate that C2 protein encoded by a geminivirus (beet severe curly top virus, BSCTV) mediated decrease repeat regions promoters ACD6, upstream regulator salicylic acid defense pathway, GSTF14, endogenous glutathione S-transferase superfamily implicated numerous stress responses. C2-mediated decreases reduced accumulation siRNAs derived from promoter repeats enhanced steady-state both ACD6 GSTF14 transcripts. Reduced BSCTV-derived was detected BSCTV-infected but not plants infected with C2-deficient BSCTV (c2(- ) BSCTV). exhibited no siRNA-binding activity. Instead, our results revealed protein-mediated appeared to affect production are required targeting reinforcing RdDM, process activated defense-related genes normally dampened these host plants. However, C2-dependent reduction virus-derived also benefits viruses disrupting feedback loop methylation-mediated antiviral silencing.