An Arabidopsis ATPase gene involved in nematode‐induced syncytium development and abiotic stress responses

• DOI: 10.1111/tpj.12170
• Publication Date: 2013-03-12T20:18:24Z
• Authors: Muhammad Amjad Ali; Stephan Plattner; Zoran Radakovic; Krzysztof Wieczorek; Abdelnaser Elashry; Florian M.W. Grun...; Moritz Ammelburg; Shahid Siddique; Holger Bohlmann
• Keywords: Syncytium

Summary The beet cyst nematode H eterodera schachtii induces syncytia in the roots of A rabidopsis thaliana , which are its only nutrient source. One gene, At1g64110 that is strongly up‐regulated as shown by RT ‐ PCR quantitative situ and promoter:: GUS lines, encodes an AAA +‐type ATP ase. Expression two related genes syncytia, At4g28000 At5g52882 was not detected or different from control root segments. Using ami RNA lines T‐ DNA mutants, we show important for syncytium development. also inducible wounding, jasmonic acid, salicylic heat cold, well drought, sodium chloride, abscisic acid mannitol, indicating involvement this gene abiotic stress responses. We confirmed using T mutants were more sensitive to chloride during seed germination growth. These developed significantly smaller response chloride. An silico analysis showed ase t1g64110 (and t4g28000 t5g52882) belong ‘meiotic clade’ proteins includes such V ps4, k atanin, s pastin MSP 1.