Whole genome profiling physical map and ancestral annotation of tobacco Hicks Broadleaf

• DOI: 10.1111/tpj.12247
• Publication Date: 2013-05-15T10:22:53Z
• Authors: Nicolas Sierro; Jan van Oeveren; Michiel J. T. van...; Florian Martin; Keith E. Stormo; Manuel C. Peitsch; Nikolai V. Ivanov
• Keywords: Polyploid; Genome size

Genomics-based breeding of economically important crops such as banana, coffee, cotton, potato, tobacco and wheat is often hampered by genome size, polyploidy high repeat content. We adapted sequence-based whole-genome profiling (WGP™) technology to obtain insight into the model plant Nicotiana tabacum (tobacco). N. assumed originate from a hybridization event between ancestors sylvestris tomentosiformis approximately 200,000 years ago. This resulted in having haploid size 4500 million base pairs, four times larger than related tomato (Solanum lycopersicum) potato tuberosum) genomes. In this study, physical map containing 9750 contigs bacterial artificial chromosomes (BACs) was constructed. The mean contig 462 kbp, calculated coverage equaled estimated size. used method for determination ancestral origin annotation WGP sequence tags. assignment agreed with available genetic map, may be investigate evolution homoeologous segments after polyploidization. generated an essential scaffold genome. propose combination mapping tag lines generally applicable elucidate polyploid species. genes their origins will enable application biotechnology plants aimed at accelerating increasing precision abiotic biotic stress resistance.