Rice SNF2 family helicase ENL1 is essential for syncytial endosperm development

• DOI: 10.1111/tpj.12705
• Publication Date: 2014-10-18T12:35:42Z
• Authors: Tomomi Hara; Hirokazu Katoh; Daisuke Ogawa; Yasuaki Kagaya; Yutaka Sato; Hidemi Kitano; Yasuo Nagato; Ryo Ishikawa; Akemi Ono; Tetsu Kinoshita; Shin Takeda; Tsukaho Hattori
• Keywords: 2. Zero hunger; 0301 basic medicine; Molecular Sequence Data; DNA Helicases; Mitosis; Oryza; Endosperm; 03 medical and health sciences; Sequence Analysis, Protein; Chromosome Segregation; Mutation; Amino Acid Sequence; RNA, Messenger; Sequence Alignment; Phylogeny; Plant Proteins

Summary The endosperm of cereal grains represents the most important source human nutrition. In addition, provides many investigatory opportunities for biologists because unique processes that occur during its ontogeny, including syncytial development at early stages. Rice endospermless 1 ( enl1 ) develops seeds lacking an but carrying a functional embryo. produces strikingly enlarged amoeboid nuclei. These abnormal nuclei result from malfunction in mitotic chromosomal segregation development. molecular identification causal gene revealed ENL encodes SNF 2 helicase family protein is orthologous to Plk1‐Interacting Checkpoint Helicase (PICH), which has been implicated resolution persistent DNA catenation anaphase. 1‐Venus (enhanced yellow fluorescent YFP )) localizes cytoplasm interphase moves chromosome arms mitosis. also detected on thread‐like structure connects separating sister chromosomes. observations indicate conservation between PICH and confirm proposed role . Although dysfunction affects karyokinesis root meristem, plants can grow field set seeds, indicating indispensability tissue‐dependent. Notably, despite wide 1/ among eukaryotes, loss function ortholog Arabidopsis CHR 24) only marginal effects results normal plant Our suggest endowed with indispensable secure extremely rapid nuclear cycle rice.