Suppressing ABA uridine diphosphate glucosyltransferase (SlUGT75C1) alters fruit ripening and the stress response in tomato

• DOI: 10.1111/tpj.13588
• Publication Date: 2017-05-08T16:12:51Z
• Authors: Yufei Sun; Kai Ji; Bin Liang; Yangwei Du; Li Jiang; Juan Wang; Wenbin Kai; Yushu Zhang; Xiawan Zhai; Pei Chen; Hongqing Wang; Ping Leng
• Keywords: Glucosyltransferases; Uridine diphosphate; Catabolism

Abscisic acid (ABA) glucose conjugation mediated by uridine diphosphate glucosyltransferases (UGTs) is an important pathway in regulating ABA homeostasis. In the present study, we investigated three tomato SlUGTs that are highly expressed fruit during ripening, and these were localized to cytoplasm cell nucleus. Among UGTs, SlUGT75C1 catalyzes glucosylation of both IAA vitro; SlUGT76E1 can only catalyze ABA; SlUGT73C4 cannot glycosylate either or IAA. Therefore, was selected for further investigation. RNA interference significantly up-regulated expression level SlCYP707A2, which encodes 8'-hydroxylase but did not affect SlNCED1, a key enzyme biosynthesis. Suppression accelerated ripening enhancing levels promoting early release ethylene. SlUGT75C1-RNAi altered genes (genes involved ethylene wall catabolism). seeds showed delayed germination root growth compared with wild-type as well increased sensitivity exogenous ABA. plants also more resistant drought stress. These results demonstrated plays crucial role ABA-mediated seed germination, responses tomato.