Spatial analysis of lipid metabolites and expressed genes reveals tissue‐specific heterogeneity of lipid metabolism in high‐ and low‐oil Brassica napus L. seeds

• DOI: 10.1111/tpj.13959
• Publication Date: 2018-05-12T00:35:17Z
• Authors: Shaoping Lu; Drew Sturtevant; Mina Aziz; Cheng Jin; Qing Li; Kent D. Chapman; Liang Guo
• Keywords: Lipidome; Oleosin; Lipid droplet

Summary Despite the importance of oilseeds to worldwide human nutrition, and more recently production bio‐based diesel fuels, detailed mechanisms regulating seed oil biosynthesis remain only partly understood, especially from a tissue‐specific perspective. Here, we investigated spatial distributions lipid metabolites transcripts involved in seeds two low‐erucic acid genotypes Brassica napus with high low seed‐oil content. Integrated results matrix‐assisted laser desorption/ionization‐mass spectrometry imaging ( MALDI ‐ MSI ) lipids situ , lipidome profiling extracts tissues, transcriptome analysis revealed complex distribution patterns transcripts. In general, it appeared that many triacylglycerol phosphatidylcholine species distributed heterogeneously throughout embryos. Tissue‐specific identified key genes de novo fatty plastid, triacylglycerols assembly droplet packaging endoplasmic reticulum ER may contribute or phenotype heterogeneity distribution. Our imply transcriptional regulation represents an important means impacting compartmentalization seeds. While much information remains be learned about intricacies accumulation distribution, these studies highlight advances come evaluating metabolism within context multiple omics level datasets.