The genome of the medicinal plant Andrographis paniculata provides insight into the biosynthesis of the bioactive diterpenoid neoandrographolide

• DOI: 10.1111/tpj.14162
• Publication Date: 2018-11-16T15:59:22Z
• Authors: Wei Sun; Liang Leng; Qinggang Yin; MeiMei Xu; Mingkun Huang; Zhichao Xu; Yujun Zhang; Hui Yao; Caixia Wang; Chao Xiong; Sha Chen; Chunhong Jiang; Ning Xie; Xilong Zheng; Ying Wang; Chi Song; Reuben J. Peters; Shilin Chen
• Keywords: Andrographis Paniculata; Labdane; Andrographolide

Summary Andrographis paniculata is a herbaceous dicot plant widely used for its anti‐inflammatory and anti‐viral properties across distribution in China, India other Southeast Asian countries. A. was as crucial therapeutic treatment during the influenza epidemic of 1919 India, still infectious disease China. produces large quantities diterpenoid lactones andrographolide neoandrographolide, their analogs, which are touted to be next generation natural medicines lung diseases, hepatitis, neurodegenerative disorders, autoimmune disorders inflammatory skin diseases. Here, we report chromosome‐scale genome sequence 269 Mb that assembled by Illumina short reads, PacBio long reads high‐confidence (Hi‐C) data. Gene annotation predicted 25 428 protein‐coding genes. In order decipher genetic underpinning biosynthesis, transcriptome data from seedlings elicited with methyl jasmonate were also obtained, enabled identification genes encoding synthases, cytochrome P450 monooxygenases, 2‐oxoglutarate‐dependent dioxygenases UDP ‐dependent glycosyltransferases potentially involved lactone biosynthesis. We further carried out functional characterization pairs class‐I ‐ II diterpene revealing ability produce diversified labdane‐related scaffolds. addition, glycosyltransferase able catalyze O‐linked glucosylation andrograpanin, yielding major active product identified. Thus, our results demonstrate utility combined genomic transcriptomic set generated here investigation production bioactive constituents important medicinal herb .