Flexible functional interactions between G‐protein subunits contribute to the specificity of plant responses

• DOI: 10.1111/tpj.14714
• Publication Date: 2020-02-08T14:41:06Z
• Authors: Swarup Roy Choudhury; Mao Li; Veronica Lee; Raja Sekhar Nandety; Kirankumar S. Mysore; Sona Pandey
• Keywords: Obligate; Epistasis

Summary Plants being sessile integrate information from a variety of endogenous and external cues simultaneously to optimize growth development. This necessitates the signaling networks in plants be highly dynamic flexible. One such network involves heterotrimeric G‐proteins comprised Gα, Gβ, Gγ subunits, which influence many aspects growth, development, stress response pathways. In as Arabidopsis, relatively simple repertoire one canonical three extra‐large Gβ subunits exists. Because proteins form obligate dimers, phenotypes lacking sole or all genes are similar, expected. However, Gα can exist either monomers complex with Gβγ, details combinatorial genetic physiological interactions different remain unexplored. To evaluate flexible, signal‐dependent their contribution toward eliciting specific response, we have generated Arabidopsis mutants combinations genes, performed extensive phenotypic analysis, evaluated results context subunit usage interaction specificity. Our data show that multiple mechanistic modes, some cases epistatic relationships, depending on between proteins. suggests that, despite limited numbers, inherent flexibility plant G‐protein provides for adaptability needed survive under continuously changing environments.