Micrografting device for testing systemic signaling in Arabidopsis

• DOI: 10.1111/tpj.14768
• Publication Date: 2020-04-14T05:43:31Z
• Authors: Hiroki Tsutsui; Naoki Yanagisawa; Yaichi Kawakatsu; Shuka Ikematsu; Yu Sawai; Ryo Tabata; Hideyuki Arata; Tetsuya Higashiyama; Michitaka Notaguchi
• Keywords: Limiting

Grafting techniques have been applied in studies of systemic, long-distance signaling several model plants. Seedling grafting Arabidopsis, known as micrografting, enables investigation the molecular mechanisms systemic between shoots and roots. However, conventional micrografting requires a high level skill, limiting its use. Thus, an easier user-friendly method is needed. Here, we developed silicone microscaled device, chip, to obviate need for training generate less stressed more uniformly grafted seedlings. The chip has tandemly arrayed units, each which consists seed pocket germination micro-path with pairs pillars hypocotyl holding. Grafting, including germination, manipulation establishment tissue reunion, performed on chip. Using evaluated effect temperature carbon source grafting, showed that 27°C sucrose concentration 0.5% were optimal. We also used investigate mechanism iron status using quadruple nicotianamine synthase (nas) mutant. constitutive iron-deficiency response nas mutant because accumulation was significantly rescued by wild-type or roots, suggesting shoot- root-ward translocation nicotianamine-iron complexes and/or essential mobilization. our will promote