Mdm‐miR858 targets MdMYB9 and MdMYBPA1 to participate anthocyanin biosynthesis in red‐fleshed apple

• DOI: 10.1111/tpj.16111
• Publication Date: 2023-01-18T08:11:06Z
• Authors: Zhiqiang Li; Wenjun Liu; Qiaojing Chen; Shuhui Zhang; Zhuoxin Mei; Lei Yu; Chen Wang; Zhiquan Mao; Zijing Chen; Xuesen Chen; Nan Wang
• Keywords: 2. Zero hunger; Anthocyanins; Plant Breeding; Gene Expression Regulation, Plant; Malus; Fruit; Humans; 3. Good health; Plant Proteins; Transcription Factors

Anthocyanins are important secondary metabolites in plants. They for human health because of their antioxidant activities and dietary intake reduces the incidence cardiovascular cerebrovascular diseases tumors. The biosynthesis anthocyanins its regulation fruits vegetables is a global research hotspot. Compared with cultivated apples, red-fleshed apple relatively new popular commodity market. Previous studies on apples have focused basis high anthocyanin content transcriptional synthesis. In present study, we mechanism microRNA-mediated post-transcriptional synthesis apples. We identified microRNA (miRNA), designated mdm-miR858, that specifically expressed flesh fruit. expression level miR858 was significantly lower than white-fleshed overexpression mdm-miR858 inhibited accumulation, whereas silencing promoted STTM858 transgenic calli. Further analyses showed targets transcription factor genes MdMYB9 MdMYBPA1 to participate accumulation apple. Our results also show MdHY5, light signaling pathway, can bind promoter inhibit transcription, thereby regulating Based our results, describe novel HY5-miR858-MYB loop involved modulation biosynthesis. These findings provide information about how plant miRNAs regulate anabolism breeding anthocyanin-rich, varieties.