Integrated mass spectrometry imaging and single‐cell transcriptome atlas strategies provide novel insights into taxoid biosynthesis and transport in Taxus mairei stems

• DOI: 10.1111/tpj.16315
• Publication Date: 2023-05-23T13:28:37Z
• Authors: Chunna Yu; Kailin Hou; Hongshan Zhang; Xueshuang Liang; Cheng Chen; Zhijing Wang; Qicong Wu; Ganlin Chen; Jiaxu He; Enhui Bai; Xinfen Li; Tingrui Du; Yifan Wang; Mingshuang Wang; Shangguo Feng; Huizhong Wang; Chenjia Shen
• Keywords: Taxoid

Taxol, which is a widely used important chemotherapeutic agent, was originally isolated from Taxus stem barks. However, little known about the precise distribution of taxoids and transcriptional regulation taxoid biosynthesis across stems. Here, we MALDI-IMS analysis to visualize mairei stems single-cell RNA sequencing generate expression profiles. A T. atlas created, providing spatial pattern cells. Cells were reordered using main developmental pseudotime trajectory provided temporal patterns in Most taxol biosynthesis-related genes primarily expressed epidermal, endodermal, xylem parenchyma cells, caused an uneven We developed strategy screen novel transcription factors (TFs) involved regulation. Several TF genes, such as endodermal cell-specific MYB47 NAC2 bHLH68, implicated potential regulators biosynthesis. Furthermore, ATP-binding cassette family transporter gene, ABCG2, proposed candidate. In summary, generated metabolic identified molecular mechanisms underpinning pathway.