Red blood cells stored for increasing periods produce progressive impairments in nitric oxide–mediated vasodilation

• DOI: 10.1111/trf.12111
• Publication Date: 2013-03-13T05:15:37Z
• Authors: Jason T. Alexander; Alexander M. El‐Ali; James L. Newman; Sulaiman Karatela; Benjamin L. Predmore; David J. Lefer; Roy L. Sutliff; John D. Roback
• Keywords: Ex vivo

Background Clinical outcomes in transfused patients may be affected by the duration of blood storage, possibly due to red cell ( RBC )‐mediated disruption nitric oxide NO ) signaling, a key regulator vascular tone and flow. Study Design Methods AS ‐1 units stored up 42 days were sampled at selected storage times. Samples added aortic rings ex vivo, system where ‐mediated vasodilation could experimentally controlled. Results showed storage‐dependent changes plasma hemoglobin (Hb), 2,3‐diphosphoglycerate acid, adenosine triphosphate conforming expected profiles. When freshly collected D ay 0) was rat rings, methacholine MCh stimulated substantial vasodilation. In contrast, produced no presence for days. Surprisingly, vasoinhibitory effects s almost totally mediated themselves: removal supernatant did not attenuate inhibitory effects, while addition alone only minimally inhibited ‐stimulated relaxation. Stored inhibit direct donor, demonstrating that mechanism work scavenging. Conclusions These studies have revealed previously unrecognized activity RBCs , which is more potent than described free Hb works through different does involve scavenging but function reducing endothelial production. Through this novel mechanism, transfusion small volumes able disrupt physiologic vasodilatory responses thereby cause adverse clinical outcomes.