Analytical validation of an enzyme‐linked immunosorbent assay for the quantification of S100A12 in the serum and feces of cats

• DOI: 10.1111/vcp.12812
• Publication Date: 2019-12-10T06:23:39Z
• Authors: Cory S. Bridges; Niels Grützner; Jan S. Suchodolski; Jörg M. Steiner; Romy M. Heilmann
• Keywords: Serial dilution

Abstract Background Measuring S100A12 concentrations in serum and feces is a sensitive specific marker of inflammation, such as seen with chronic gastrointestinal inflammation people dogs. Biomarkers cats are currently lacking. Objectives We aimed to analytically cross‐validate the canine S100A12‐ELISA for measurement feline specimens. Methods The ELISA was validated by assessing dilutional linearity, spiking/recovery, intra‐ inter‐assay variability. Reference intervals fecal were calculated using samples from healthy cats, short‐term biological variation assessed. Results Observed‐to‐expected ratios (O/E) serial dilutions extracts ranged 91%‐159% (mean, 120%) 100%‐128% 114%), spiking/recovery method 106%‐263% 154%) 52%‐171% 112%). Intra‐ CV% ≤5.6% ≤14.0%, ≤3.8% ≤19.1%, repsectively. RIs <43 µg/L < 20 ng/g, respectively. A mild biologic variation, but large individuality detected when measuring cats. Conclusions accurate, reproducible, sufficiently linear precise samples. use this assay reasonable option specimens provides basis further evaluation disease. Using population‐based RI appears be limited value.