The effect of pH on cell viability, cell migration, cell proliferation, wound closure, and wound reepithelialization: In vitro and in vivo study

• DOI: 10.1111/wrr.12526
• Publication Date: 2017-04-04T14:20:41Z
• Authors: Carla R. Kruse; Mansher Singh; Stefan Targosinski; Indranil Sinha; Jens A. Sørensen; Elof Eriksson; Kristo Nuutila
• Keywords: Viability assay; Wound Closure

Abstract Wound microenvironment plays a major role in the process of wound healing. It contains various external and internal factors that participate pathophysiology. The pH is an important factor influences healing by changing throughout process. Several previous studies have investigated relation to pathogens but concentrating on effects itself are inconclusive. purpose this study was comprehensively controlled fashion investigate effect studying its human primary keratinocyte fibroblast function vitro vivo. In vitro, keratinocytes fibroblasts were cultured different levels (5.5–12.5) cell viability, proliferation, migration studied. A rat full‐thickness model used (5.5–9.5) inflammation monitored measuring IL‐1 concentrations from wounds cultures exposed environments. Our results showed both skin types tolerated wide range very well. They further demonstrated acidic alkaline environments decelerated comparison neutral interestingly conditions significantly enhanced proliferation. Results vivo experiments indicated prolonged, strongly environment prevents closure reepithelialization while prolonged did not any negative impact or reepithelialization. Separately, increased expression fluid, whereas result elevated amounts .