Molecular phylogeny of the nematode genusLongidorus(Nematoda: Longidoridae) with description of three new species

• DOI: 10.1111/zoj.12019
• Publication Date: 2013-03-27T11:04:48Z
• Authors: Carlos Gutiérrez-...; Carolina Cantalap...; Miguel Montes-Bor...; Juan E. Palomares...; Pablo Castillo
• Keywords: 0303 health sciences; 03 medical and health sciences

The phylum Nematoda includes the genus Longidorus, a remarkable group of invertebrates that are polyphagous root-ectoparasites many plants including various agricultural crops and trees. Damage is caused by direct feeding on root cells as well transmitting nepoviruses. Species discrimination in Longidorus complicated phenotypic plasticity (intraspecific variability minor interspecific differences) leading to potential misidentification. We conducted nematode surveys cultivated natural environments southern Spain detected 11 species Longidorus. developed comparative study amongst these related considering morphological morphometric features together with molecular data from nuclear ribosomal RNA genes [D2-D3 expansion segments large subunit (28S), internal transcribed spacer 1 (ITS1), partial small (18S)]. results our phylogenetic analyses confirmed hypotheses allowed delimitation three new genus, described herein baeticus sp. nov., oleae andalusicus eight known (Longidorus alvegus, crataegi, fasciatus, intermedius, iuglandis, magnus, rubi, vineacola). Phylogenetic spp. based markers resulted general consensus grouping, lineages were maintained for majority (i.e. conoid-rounded lip region, amphidial fovea asymmetrically bilobed, female tail bluntly rounded), but not some others positions L. rubi quite variable). To date, this most complete analysis Paralongidorus species, highest number included. No correspondence between trees characters was found markers, exception shape. Thus, polyphasic identification, integration morphology, tool beyond doubt identification.