Pannexin 1 channels mediate the release of ATP into the lumen of the rat urinary bladder

• DOI: 10.1113/jphysiol.2014.283119
• Publication Date: 2015-01-29T16:49:08Z
• Authors: Jonathan M. Beckel; Stephanie L. Daug...; Pradeep Tyagi; Amanda S. Wolf‐Jo...; Lori A. Birder; Claire H. Mitchell; William C. de Groat
• Keywords: Pannexin; Adenosine triphosphate; Lumen (anatomy)

ATP is released through pannexin channels into the lumen of rat urinary bladder in response to distension or stimulation with bacterial endotoxins. Luminal plays a physiological role control micturition because intravesical perfusion apyrase ecto-ATPase inhibitor ARL67156 altered reflex activity anaesthetized rat. The release from apical and basolateral surfaces urothelium appears be mediated by separate mechanisms administration channel antagonist Brilliant Blue FCF increased capacity, whereas i.v. did not. Intravesical instillation small interfering RNA-containing liposomes decreased 1 expression vivo capacity. These data indicate for pannexin-mediated luminal both pathophysiological suggest that urothelial may viable target treatment overactive disorders.ATP epithelium, also termed urothelium, mechanical chemical stimuli. Although numerous studies have described contribution this development various disorders, little information exists regarding release. In present study, we examined mechanically-induced urothelium. PCR confirmed presence 2 mRNA tissue, immunofluorescence experiments localized all three layers During continuous cystometry rats, inhibition using carbenoxolone (CBX) (BB-FCF) (1-100 μm, intravesically), RNA, interval between voiding contractions. Intravenous BB-FCF μg kg(-1) ) not alter activity. CBX (100 μm intravesically) basal concentrations perfusate non-distended bladders inhibited increases (15 30 cmH2 O pressure). diphosphohydrolase (2 U ml(-1) ), ATPase (10 μm) activity, respectively. lipopolysaccharides (LPS) (Escherichia coli 055:B5, 100 perfusate, frequency; these effects were suppressed BB-FCF. contribute distension- LPS-evoked can modulate function.