Molecular identification of seven species of the genus Stigmaeopsis (Acari: Tetranychidae) and preliminary attempts to establish their phylogenetic relationship

• DOI: 10.11158/saa.22.1.10
• Publication Date: 2017-01-04T23:59:16Z
• Authors: Hironori Sakamoto; Tomoko Matsuda; Reiko Suzuki; Yutaka Saito; Jian-Zhen Lin; Yan-Xuan Zhang; Yukie Sato; Tetsuo Gotoh
• Keywords: Monophyly; Nuclear gene; Molecular Phylogenetics

The genus Stigmaeopsis (family Tetranychidae) has 11 species including the serious bamboo pest, S. nanjingensis. All are difficult to identify by their morphology, and diagnostic character (the length of dorsal setae) can be used only fresh specimens. To these at molecular level, we sequenced cytochrome c oxidase subunit I (COI) gene mitochondrial DNA two nuclear ribosomal RNA genes (18S 28S) 20 strains seven [S. celarius, longus, miscanthi (both low- high-aggression phenotypes), nanjingensis, tenuinidus, saharai takahashii]. In maximum likelihood (ML) phylogenetic trees both COI combined 18S-28S genes, all but one could identified as a monophyletic clade with high bootstrap values. present results strongly suggested that exceptional species, miscanthi, consists three biologically different entities based on trees. Though did not comprehensively solve phylogeny Stigmaeopsis, tree showed sibling relationship between sub-social longus. addition, PCR detected Wolbachia or Cardinium, which frequently affect haplotypes, in longus tree, was separated into groups were more consistent bacterial infection status than geographical distribution.