Programmable scanning diffuse speckle contrast imaging of cerebral blood flow

• DOI: 10.1117/1.nph.12.1.015006
• Publication Date: 2025-01-27T08:29:17Z
• Authors: Faezeh Akbari; Xuhui Liu; Fatemeh Hamedi; Mehrana Mohtasebi; Li Chen; Lei Chen; Guoqiang Yu
• Keywords: Temporal resolution

Significance: Cerebral blood flow (CBF) imaging is crucial for diagnosing cerebrovascular diseases. However, existing large neuroimaging techniques with high cost, low sampling rate, and poor mobility make them unsuitable continuous longitudinal CBF monitoring at the bedside. Aim: This study aimed to develop a low-cost, portable, programmable scanning diffuse speckle contrast (PS-DSCI) technology fast, high-density, depth-sensitive of in rodents. Approach: The PS-DSCI employed digital micromirror device (DMD) remote line-shape laser (785 nm) on tissue surface synchronized 2D camera capturing boundary contrasts. New algorithms were developed address deformations scanning, thus minimizing reconstruction artifacts. was examined head-simulating phantoms adult mice. Results: enables resolving Intralipid particle contrasts different depths. In vivo experiments mice demonstrated capability image global/regional variations induced by 8% CO2 inhalation transient carotid artery ligations. Conclusions: Compared conventional point line significantly increases spatiotemporal resolution. rate rapid changes while spatial resolution important visualizing brain vasculature.