Establishing the diffuse correlation spectroscopy signal relationship with blood flow

• DOI: 10.1117/1.nph.3.3.031412
• Publication Date: 2016-06-13T03:32:28Z
• Authors: David A. Boas; Sava Sakadžic; Juliette Selb; Parisa Farzam; Maria Angela Fran...; Stefan A. Carp
• Keywords: Photon diffusion; Fluorescence Correlation Spectroscopy

Diffuse correlation spectroscopy (DCS) measurements of blood flow rely on the sensitivity temporal autocorrelation function diffusively scattered light to red cell (RBC) mean square displacement (MSD). For RBCs flowing with convective velocity [Formula: see text], is expected decay exponentially where text] delay time. also experience shear-induced diffusion a coefficient and an MSD text]. Surprisingly, experimental data primarily reflect diffusive behavior. To provide quantitative estimates relative contributions movements, we performed Monte Carlo simulations scattering through tissue varying vessel densities. We assumed laminar profiles accounted for effects. In agreement data, found that motion dominates typical DCS measurement parameters. Furthermore, our model offers relationship between RBC absolute flow. thus offer, first time, theoretical support empirically accepted ability index ([Formula: text]) quantify perfusion. find be linearly proportional flow, but proportionality modulated by hemoglobin concentration average diameter.